CD Genomics offers highly flexible shotgun library construction services, including shotgun sequencing of the completed library. High quality random-sheared shotgun libraries of BAC clones and whole genomes are regularly produced at CD Genomics. Our shotgun library construction has been done with a variety of vectors such as plasmid, cosmid, fosmid, BAC, and YAC.

Construction Steps:

•   • Plasmid DNA purification and/or quality analysis of in-coming DNA

•   • Random shearing to produce a population of DNA fragments

•   • Ligation into a standard cloning vector and transformation

•   • Libraries submitted to customer as plates, glycerol stocks, or purified DNA

•   • QC by PCR or DNA sequencing 

http://www.cd-genomics.com/Shotgun-library-construction-service.html