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BAC libraries are essential elements in doing large-scale genome research such as genome sequencing, physical maps, and gene cloning. This technology was used in applications for genomic analysis that included large-scale physical mapping and genomic sequencing although this technology was developed much later than Yeast Artificial Chromosome (YAC) and cosmid cloning technologies.
This service should be attributed to the technical advantages of BAC cloning over YAC and cosmid cloning system summarized as follows:
• Bigger insert size (up to 300 kb) than cosmid and other plasmid system.
• Much more stable than yeast.
• Bacterial clones and libraries grow much faster than YAC.
• Easier in handling and gridding during DNA preparation.
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